Mar 27, 2015 the potential use of sporosarcina pasteurii in possible biotechnological applications on a large scale ground improvement, consolidation of building structures and ornamental stone, or in developing biomaterials for the building industry, is based on its ability to produce high amounts of carbonate in a short period of time via urea hydrolysis. Pdf biocementation of sand by sporosarcina pasteurii strain. Pdf strain improvement of sporosarcina pasteurii for. Pdf strain improvement of sporosarcina pasteurii for enhanced. Biocementation of sand by sporosarcina pasteurii strain and. Bioprecipitation of calcite by sporocarcina pasteurii. Microbiologically induced calcite precipitation mediated by. Developing efficient methodologies for microbially indurated rammed earth by joshua w. Application of an indigenous bacterium in comparison with.
Using microfluidic setup to determine the adsorption rate of. Microbial induced calcium carbonate precipitation at elevated. Sporosarcina pasteurii click on organism name to get more information. The bacterium sporosarcina pasteurii sp is known for its ability to cause the. To investigate potential implications of microbial activity on sand bioconsolidation and subsurface environments, two ureolytic strains, sporosarcina pasteurii and bacillus subtilis were tested for the production of calcium carbonate caco3. Sporosarcina ureae is a type of bacteria of the genus sporosarcina, and is closely related to the genus bacillus. Alternative nutrient sources for biotechnological use. Crystals free fulltext comparative study on the sand.
A bacterial transport model was developed to fit the breakthrough curve data from s. Phylum firmicutes, class bacilli, order bacillales, family planococcaceae, genus sporosarcina, sporosarcina pasteurii yoon et al. Sep 14, 2015 firmicutes bacilli bacillales planococcaceae sporosarcina e. Sporosarcina pasteurii is a bacterium which is able to break down the urea. Production of urease enzyme from ureolytic bacteria e. Boling submitted to the graduate degree program in geology and the graduate faculty of the university of kansas in partial fulfillment of the requirements for the degree of master of arts. Moreover, in the bulk agar we found the presence of microspheres, which seem to. It was determined that over the time frame of the experiment, that b. The bacteria degraded the urea present in the urine to form carbonate. Sporosarcina pasteurii ptcc1645 was used as a urease positive bacterium. The bacterium sporosarcina pasteurii can produce significant volumes of solid precipitation in the presence of specific chemical environments. Description of paenisporosarcina quisquiliarum gen. The fit of the ligand to the active site involves stabilizing interactions, such as a carboxylate group that binds the nickel ions at the active site and several. Sporosarcina globispora w 25 t dsm 4 is a grampositive, roundsporeforming, and psychrophilic bacterium.
Abstract phenotypic mutants of sporosarcina pasteurii. Our scientists ensure that the identification of the cultures is correct and they remain unchanged from when they are first deposited with the collection. Application of carbonate precipitation induced by bacillus pasteurii for. The ncbi taxonomy database is not an authoritative source for nomenclature or classification please consult the relevant scientific literature for the most reliable information. This bacterium exists in the soil naturally and can also be isolated from soil if. Biocementation of sand by sporosarcina pasteurii strain and technicalgrade cementation reagents. Urease is a virulence factor in several human pathogens, while causing severe environmental and agronomic problems.
Some bacteria like sporosarcina pasteurii have the unique capability to secrete large volumes of urease, an enzyme that catalyzes the hydrolysis. This sequence was repeated over eight hours for a total of 800mg added urea. The high activity of urease, a niii enzyme, has several adverse effects on human health and agriculture, and its modulation needs the use of inhibitors. The hydraulic conductivity of soil columns treated with s. Sporosarcina pasteurii can clog and strengthen a porous medium. Due to the rodshaped cells and the ability to produce endospores, it was assigned to the genus bacillus in the past. Sporosarcina pasteurii can clog and strengthen a porous. Jan 23, 2020 the ureolytic bacterium sporosarcina pasteurii is wellknown for its capability of microbially induced calcite precipitation micp, representing a great potential in constructional engineering and material applications. Sporosarcina pasteurii is a grampositive bacterium able to survive in highly alkaline environments ph10 1 and is one of the bacterial species that can become a causative agent of a phenomenon called microbiologically induced calcite precipitation micp 24.
Jun 23, 2010 corn steep liquor csl, an industrial byproduct, was used as a nutrient source to grow sporosarcina pasteurii, a bacterium used to remediate cracks and fissures in building materials and structures. Biocementation of sand by sporosarcina pasteurii strain and technicalgrade cementation reagents through surface percolation treatment method. Whole cell kinetics of ureolysis by sporosarcina pasteurii. Pdf sporosarcina pasteurii can form nanoscale calcium carbonate. Pdf alternative nutrient sources for biotechnological use. Manufacturing biobricks using microbial induced calcium carbonate. Pdf phenotypic mutants of sporosarcina pasteurii previously known as.
This concept is also known as microbiologically induced calcite. Beneficial factors for biomineralization by ureolytic bacterium. Pdf the bacterium sporosarcina pasteurii sp is known for its ability to cause the phenomenon of microbially induced calcium. A cost effective cultivation medium for biocalcification of bacillus. Strain improvement of sporosarcina pasteurii for enhanced urease and calcite production article pdf available in journal of industrial microbiology 367. Our platform allows for insitu visualization of the phenomena, and we found clear evidence of bacterial cell surface facilitating formation of nanoscale crystals. Kinetic and thermodynamic studies of chromium vi biosorption on sporosarcina pasteurii. Pdf alternative nutrient sources for biotechnological. Sporosarcina pasteurii can form nanoscale crystals on cell.
Microbiologically induced calcite precipitation mediated by sporosarcina pasteurii. Selectivity of niii and znii binding to sporosarcina pasteurii uree, a metallochaperone in the urease assembly. To date, no researcher has investigated the applicability of sporosarcina pasteurii bacteria to survive at elevated ph values. The culture collections hold cell cultures, bacteria, fungi and virus strains from worldwide sources. Sporosarcina pasteurii sp, formerly bacillus pasteurii, is a non. Draft genome sequence of sporosarcina globispora w 25t dsm 4. Lowcost cultivation of sporosarcina pasteurii strain in food. Comparative study on the sand bioconsolidation through calcium. Sporosarcina pasteurii is essential for precipitation of caco3. Bacillus pasteurii miquel chester 1898, urobacillus pasteurii miquel 1889, bacillus probates vihoever 19. We explored bacterial participation in the initial stages of the micp process at the cellular length scale under two different growth environments a liquid culture b micp in a soft agar 0. The addition of urea into the culture medium provided an.
Pdf beneficial factors for biomineralization by ureolytic. Here, we determined breakthrough curves of sporosarcina pasteurii in bentheimer sandstone as a function of core length, bacterial density, and flow rate. Urease is the most efficient enzyme known to date, and catalyzes the hydrolysis of urea using two niii ions in the active site. Micromorphological and biocalcification effects of sporosarcina pasteurii and sporosarcina ureae in sandy soil columns. Original article whole cell kinetics of ureolysis by sporosarcina pasteurii e. Strain improvement of sporosarcina pasteurii for enhanced urease and calcite production. We used sporosarcina pasteurii bacteria to help drive the micp process. Optimized carbonate microparticle production by sporosarcina pasteurii using response surface methodology.
Sporosarcina pasteurii formerly known as bacillus pasteurii from older taxonomies, is a bacterium with the ability to precipitate calcite and solidify sand given a calcium source and urea, through the process of microbiologically induced calcite precipitation or biological cementation. Alternative nutrient sources for biotechnological use of sporosarcina pasteurii. Sporosarcina pasteurii urease has been used extensively in the structural characterization of the. In the present article, we describe a novel method to measure the adhesion rate of sporosarcina pasteurii bacteria on sandstone and that, additionally, allows to obtain information about local position of the calcite crystals on the sandstone grains. However, the molecular mechanism for its biomineralization remains unresolved, as few studies were carried out. Biological precipitation of calcium carbonate using sporosarcina pasteurii, has been developed in the recent decades for the civil applications such as improvement of soil engineering parameters, crack repair in concrete and remediation of contaminated soil. The bacterium sporosarcina pasteurii sp is known for its ability to cause the phenomenon of microbially induced calcium carbonate precipitation micp. The ureolytic bacterium sporosarcina pasteurii is wellknown for its capability of microbially induced calcite precipitation micp, representing a. Transport of sporosarcina pasteurii in sandstone and its significance for subsurface engineering technologies. The taxonomic positions of two grampositive, endosporeforming rods, strains ccug 53915t and ccug 53480t, isolated from an industrial cleanroom floor and from a human blood sample, respectively, were studied. Sporosarcina major reference works wiley online library. Here, kinetic studies on the fluorideinduced inhibition of urease from sporosarcina pasteurii, a widespread and highly ureolytic soil bacterium, were performed using isothermal titration calorimetry and revealed a mixed competitive and uncompetitive mechanism.
These solid precipitate particles can enter a network of microscale pores and cause longrange clogging. The method is based on the detection of the crystals developed on grains placed inside a. Whole cell kinetics of ureolysis by sporosarcina pasteurii e. Alternative nutrient sources for biotechnological use of. Sporosarcina pasteurii can form nanoscale calcium carbonate. German collection of microorganisms and cell cultures gmbh. The ureolytic bacterium sporosarcina pasteurii is wellknown for its capability of microbially induced calcite precipitation micp. Sporosarcina pasteurii is able to produce in certain culture media calcium carbonate, this makes them interesting for applications in. Sporosarcina pasteurii was chosen because it is a alkaline microorganism, nonpathogenic and with high levels of intracellular. Sporosarcina pasteurii bacteria has commonly been used for micp due to its ability to produce high amounts of the enzyme urease and because it is a nonpathogenic species.
Pdf kinetic and thermodynamic studies of chromium vi. Pdf micromorphological and biocalcification effects of. Pdf microbiologically induced calcite precipitation. Beneficial factors for biomineralization by ureolytic. For this reason, it is proposed that strains ccug 53915 t dsm 22204 t and ccug 53480 t dsm 22203 t represent two novel species in the genus sporosarcina, with the names sporosarcina contaminans sp. Leibniz institute dsmzgerman collection of microorganisms and cell cultures gmbh. Sporosarcina pasteurii is a wellknown ureolytic microbial species that proficiently induces the deposition of calcium carbonate through microbially induced carbonate precipitation micp process for various biotechnological and engineering purposes. Pdf optimized carbonate microparticle production by. Microcontinuum modelling of injection strategies for microbially. Effects of different calcium salts on calcium carbonate crystal formation by sporosarcina pasteurii kctc 3558. Transport of sporosarcina pasteurii in sandstone and its. As a result, the medium gains strength and exhibits superior mechanical properties.
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